Rabbit & Rodent Diagnostic Associates


Services for routine mycological culture for isolation and identification of fungi areavailable.


Choice of Sample Site
Use of a UV light can be a helpful adjunct in the selection of infected hairs for culture. Choose fluorescing hairs and skin fragments. The periphery of active lesions is the best area to obtain the samples. Hairs that are fluorescent, distorted, or fractured should be cultured. Culture of the basal portion of infected hair is recommended. Place at least 10-12 hairs in a sterile screw-cap container.

Preparation of Collection Site
To prevent bacterial interference with laboratory evaluation for fungi, the collection site should be cleaned if grossly contaminated. Soap and water may be used -- gently, to avoid mechanical removal of infected material. A gauze sponge soaked in 70% alcohol may be laid over the sample site for 30 seconds or wiped gently over the site. Let the site dry before collecting the sample. Avoid iodine or other fungicidal preps, as they may effect sterilization of the sample.

Cutaneous Specimens
Hair Specimen
Sample Storage
Additional Information Histoplasmosis, sporotrichosis, coccidioidomycosis, cryptococcosis, and phycomycosis usually do not pose significant problems in laboratory animals, but they may disrupt research in animals that are immunosuppressed
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