Gene Therapy
- Determine the status of the integration of constructs into a host genome
- Determine the efficiency.
- Determine the chromosomal location of the constructs and the number of integrating sites.
- Determine the specificity of integration: random or non-random integration in the host genome
- Determine the level of integrity a construct maintains after transfection.
- Determine the size and number of the inserted constructs in the same insertion site
- Determine the stability of integration after varying periods of cell division
- Trace sub-populations of cells after integration
- Determine the correlation between integration sites and the level of gene expression