Gene Mapping on Chromosomes

• Novel gene or DNA fragment mapping on banded chromosomes (human, mouse, and rat)
• Cross species mapping utilizing alternate species cDNA probes. This can be done, for example, by mapping a human locus using mouse, rat, other mammalian and even fly cDNA.
• Evaluate syngeneic relationships between human, mouse, rat and other species
• Confirm EST information
• Confirm mapping data generated from a radiation hybrids panel
• Deletion/amplification studies of a novel gene in tumor cell lines
• Mapping on DAPI banded chromosomes is effective for chromosome identification and for the precise mapping.
• Combine metaphase orientation and interphase distance measurements to make long-range physical maps of multi-megabase chromosomal regions.
• Map the linear orientation of two or more clones on metaphase chromosomes using two-color or multi-color FISH.
• Hybridize two clones known to be located on the same chromosome in two colors to interphase nuclei and measure the distance between them in kilobases.