ELISPOT for spleen, lymph node or PBMC
Th-1 and/or Th-2 cytokine expression,  i.e.:
IL2 IL4, IFNγ

Intracellular cytokine staining FACS (spleenor lymph node or PBMC), i.e.:
IL2 IL4, IFNγ

Cytokine release by ELISA (spleen, lymph nodes or PBMC) i.e.:
IL2 IL4, IFNγ

T-cell stimulation/proliferation assays (spleen, lymph node or PBMC)

T cell subset: i.e. CD4, CD8, NK cells, macrophages, dendritic cells, etc

Mixed leukocyte response (MLR) against allogeneic leukocytes

T-cell co-cultured with dendritic or other Antigen Presenting Cells (APC) cells:

• Delayed-type hypersensitivity response to sheep RBCs or other such as chicken RBCs
• Skin Rash
• Lymphadenopathy
• Urticaria (Hives)
• IgE