PATHOLOGY AND FOLLOW-ON STUDIES

Once tissues have been collected, there are a variety of follow-on studies that can be performed. Our capabilities include:

• Basic histology (H&E stain)
• Use of special stains (Masson trichrome, etc.)
• Proliferation and apoptosis immunostains
• Immunohistochemistry for both monoclonal and polyclonal antibodies
• Fluorescent immunohistochemistry
• Cellular hypoxia measurements
• Blood vessel density measurements
• Biochemical assays for target effectiveness on treated animal tissues

Molecular biology is an integral tool for analyzing treated tissues, building molecular probes and developing new animal models. Some of our expertise includes:

• DNA, RNA and protein extraction from cells and tissues
• Western, Northern and Southern blot analysis, immunoprecipitation and kinase assays
• Gene construction, transfection, and expression
• DNA sequencing and mutation detection
• cDNA synthesis, primer design, PCR, and RT-PCR
• Genotyping, DNA enzymology, plasmid DNA sub-cloning
• Developing knockout and transgenic mice.