Rabbit & Rodent Diagnostic Associates

Paraffin Sections

All paraffin embedded tissue is cut at a thickness of 3-5 m. Thicker sections make morphological assessment much more difficult. The sections are floated on a warm water bath (45C), before being picked up onto microscope slides and allowed to drain. Sections for tinctorial staining are placed on a hot plate (56C) for 15 minutes before staining. Sections for immunocytochemical staining are picked up on aminopropyltriethoxysilane (APES) coated slides and dried overnight in an incubator at 37C.

One section cut from each paraffin block is stained with haematoxylin and eosin (H&E). After initial examination of the H&E section either additional tinctorial stains (for example, Gordon and Sweet's reticulin stain) or specific panels of immunocytochemical markers may be performed.
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