Efficacy Evaluation of Cancer Drugs

In Vitro Efficacy Evaluation of Cancer Drugs

MDS offers a variety of in vitro assays to evaluate a test article's effects on tumor cell biology, including proliferation, cytotoxicity, apoptosis and cell cycle analysis. We also perform functional cell receptor binding and activity assays and can examine a compound’s effects on migration and invasion (Matrigel and fibrin gel). We offer several assays to evaluate a compound's effects on angiogenesis, including endothelial proliferation, migration and tube formation studies as well as the chick chorioallantoic membrane (CAM) assay.

MDS offers a number of pro- and anti-angiogenic assays:

• Both the pro- and anti-angiogenic effects of potential new drugs
• Ex vivo chick chorioallantoic membrane (CAM) assay
• Endothelial cell proliferation, migration, differentiation, and tube assays
• Formation assays

Chemotaxis: Human vascular endothelial cell culture (HUVEC) populations are very responsive to compounds that modulate angiogenic response. Pro-angiogenic compounds promote increased growth and differentiation rates in these cells. Activated endothelial cells become highly chemotactic. We can evaluate the effect of compounds on the growth rate of HUVEC cells and, by using Boyden chambers, a compound's ability to induce migration through a defined matrix.