Cell Based Assays
Hemolytic Potential and Compatibility
- Hemolytic Potential in Rat or Dog Blood: Whole rat or dog blood is exposed to the test article and the amount of hemoglobin released from lysed cells is determined spectrophotometrically. Untreated controls, vehicle controls, and positive controls are included. Known volumes of whole blood are used to generate a standard curve representing 0% to 100% hemolysis.
- Hemolytic Potential in Human Blood: Same as above but using human blood
- Hemolytic Potential in Rat, Dog, and Human Blood: Same as above but using blood from all three species
- Optional Plasma and Serum Compatibility: Concurrent with above, compatibility is determined by adding the test article to plasma and serum individually and then assessing the occurrence or non-occurrence of precipitation or coagulation of plasma or serum proteins.
Isoenzyme analysis can determine the species identity of your cell line and determine if your cell line has been contaminated by a cell line of a different species. From the banding patterns of the enzymes used, a "process of elimination" is employed to determine the species identity of the cell line.
Standard isoenzyme analyses include determination of the isoenzyme gel electrophoresis banding pattern for at least three of the following enzymes:
- Glucose-6-Phosphate Dehydrogenase (G6PD)
- Lactate Dehydrogenase (LD)
- Malate Dehydrogenase (MD)
- Nucleoside Phosphorylase (NP)
- Aspartate Aminotransferase (AST)
- Peptidase B (PepB)
- G-Protein Coupled Receptors (GPCR)
- cAMP Phosphodiesterase Assay
- Protein Kinase Assay (PK)