Alkaline Phosphatase - Anti-Alkaline Phosphatase (APAAP) Technique

The APAAP technique is an indirect method which utilizes a pre-formed, cyclic enzyme anti-enzyme immuno complex composed of three enzyme molecules (alkaline phosphatase) and two antibody molecules. The technique can be visualized by using either a blue dye (Fast Blue BBN) or a red dye (Fast Red TR). The APAAP technique can be used as an alternative to peroxidase techniques and is particularly useful for double labeling techniques and for antigen demonstration in slides rich in endogenous peroxidase activity, namely peripheral blood and bone marrow aspirate specimens.