Enzyme Linked Immunosorbent Assay (ELISA)
- Direct ELISA
- Competitive Enzyme Immunoassays (small molecules)
- Immunometric/Sandwich Assays (large molecules)
- ELISA formats (immunoglobulins)
- Particle Based
- Immuno-capture enzymatic assay
- Colloidal gold immuno-chromatography strip
- Feasibility assessment and assay design
- Assay optimization
- Assay Validation
- Manufacturing
- Colorimetric » Moderate sensitivity
- Fluorescent » High sensitivity
- Bioluminescent » Very high sensitivity
- Chemiluminescent » Highest sensitivity
- Fluorescence resonance energy transfer
Custom Reagent Kit Manufacturing:
MDS has the ability to custom manufacture assay kits in virtually any lot size. Multiple packaging options are available. Our scientists are prepared to fully accommodate our clients' needs for development of raimmunoassay kits for quantitative detection of enzymes, antibodies, antigens, receptors, inhibitors, peptides and small molecules from cell culture supernatant, tissue homogenate, cell lysate, serum, plasma, ascites, cerebrospinal fluid and urine. We can also build up kits that are intended for analysis of enzyme activity, protein-protein interaction, nucleic acid-protein interaction and inhibitor characterization.
On a custom basis, we can pre-coat plates in a quality controlled environment to ensure the reproducibility you demand. Let our experts create unique plates to support your trials or other higher volume work.
ELISA ASSAYS for DIABETES & OBESITY (Human, Rat, and Mouse)
| GLP-1 (Active) | Adiponectin |
| Diponectin | Ghrelin-Desacyl |
| Amylin (Total) | Amylin |
| Insulin | Human ProInsulin |
| Leptin | Resistin |
| C-Peptide | Osteocalcin |
| Chemokines | Cyclic nucleotides |
| Cytokines | Drug/Drug metabolites |
| Enzyme activity | Immunoglobulins |
| Nitricoxide | Corticosterone |
| Steroids | Angiotensin Converting Enzyme (ACE) |
| PTN | EPO |
Isotyping of Immunoglobulin Classes and Subclasses:
Quantitation of the major isotypes (IgG1, IgG2b, IgG3, IgA, IgM, IgD, and IgE) in the serum is accomplished using radial immunodiffusion and ELISA tests.