HLA Typing
HLA typing is a part of genotyping program at MDS. The assay is designed to determine HLA matching between cell lines and to confirm HLA identity for related cell based samples by sequencing, SSO, SSP and/or Cloning to resolve ambiguities. HLA comprises two classes:
- Class I: Three loci A, B, C are the most significant loci (other loci eg E,F,G,H etc are not so important in tissue typing transplantation). There are expressed on most nucleated cells.
- Class II: Five loci DR, DQ, DP, DM and DO. HLA-DR, DQ, DP are the most significant. These are expressed on B lymphocytes, activated T lymphocytes, macrophages,
MDS Offers the High, Intermediate and Low Allele Resolutions
High resolution typing for the following loci:
- HLA-A HLA-DRB1 HLA-DPB1
- HLA-B HLA-DRB3/4/5 HLA-DQA1
- HLA-C HLA-DQB1 HLA-DPA1
Intermediate resolution typing is available for the following loci:
- HLA-A HLA-DRB1
- HLA-B HLA-DQB1
- HLA-C HLA-B27
Low resolution typing is available for the following loci:
- HLA-A
- HLA-B
- HLA-C
- Genomic DNA – 2ugDNA
- Whole blood - 4ml fresh whole blood drawn into EDTA
- Buffy coat derived from fresh whole blood 1 mL
- Cryopreserved cells: Vial of 1-2x106 cryopreserved