Recommended Sample Size for PCR
Sample |
Amount* |
Cultured cells |
1 x 10^7 |
Liver |
25 mg (~2mm3) |
Brain+ |
25 mg |
Lung+ |
25 mg |
Heart/muscle+ |
25 mg |
Kidney |
25 mg |
Spleen+ |
10 mg |
Plant leaf |
100 mg |
Mouse tail |
0.8-1.2 cm |
Blood |
1 ml |
Buffy coat |
50 ul |
DNA (PCR) |
500 ng/rxn |
RNA (PCR) |
500 ng/rxn |
DNA (Q-PCR) |
50 ng/rxn |
RNA (Q-RT-PCR) |
100 ng/rxn |
+These are difficult tissues and may require special processing.
Recommended Shipping Conditions
Plasmid DNA |
Liquid = A, W, D; pellet A |
PCR products |
Liquid = A, W, D; pellet A |
Primers |
Liquid = A, W, D; pellet A |
CDNA or genomic libraries |
With DMSO = D; without DMSO = W |
RNA |
D |
Bacterial colonies |
A, W |
Stab culture |
A |
Cell banks |
D |
Glycerol stocks |
D |
Tissues |
D |
Blood |
W |
A = ambient temperature
W = wet/blue ice
D = dry ice
To place an order for Real-Q-Time PCR, please click on the forms tab above and complete any/all of the pertinent submission forms.