Sample Preparation (Frozen Tissues)

• Fix tissue by vascular perfusion with 500 - 700 mL Fixation Buffer.
• Perfuse tissue with 400 mL Sucrose Solution.
• Cut tissue using a cryostat at a nominal thickness of 5 - 15 µm and thaw-mount onto histological slides.
• Dry slides with tissue sections for 30 minutes on a slide warmer at 37° C.
• Store slides in a freezer at -20 to -70° C.