Reagents

• Fixative: 4% formaldehyde (Catalog # P6148; Sigma) and of 14% (v/v) saturated picric acid in 0.16 M phosphate buffered saline (PBS), pH 6.9
• Sucrose Solution: 10% sucrose solution in 0.1 M PBS (pH 7.2)
• Incubation Buffer: 0.1 M PBS, pH 7.4 containing 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton X-100 and 0.01% sodium azide
• Wash Buffer: 0.1 M PBS, pH 7.4
• Avidin-Biotin Blocking Kit: (SP-2001, Vector Labs)
• Vectastain Elite ABC-Peroxidase Kit: (Cat# PK-6105; Vector Labs)
• Substrates: AEC (Cat# SK-4200; Vector Labs), DAB (SK-4100), VIP (SK-4600), NovaRED (SK-4800, Vector Labs)
• Mounting Media: Use an aqueous or a non-aqueous mounting media of choice. For Fluorescence staining use an anti-fade mounting media of choice