Isoenzyme Analysis
Isoenyzyme analysis can determine the species identity of your cell line and determine if your cell line has been contaminated by a cell line of a different species. From the banding patterns of the enzymes used, a "process of elimination" is employed to determine the species identity of the cell line.
Standard isoenzyme analyses included determination of the isoenzyme gel electrophoresis banding pattern for at least three of the following enzymes:
- Glucose - 6 - Phosphate Dehydrogenase (G6PD)
- Lactate Dehydrogenase (LD)
- Malate Dehydrogenase (MD)
- Nucleoside Phosphorylase (NP)
- Aspartate Aminotransferase (AST)
- Peptidase B (PepB)
- G-Protein Coupled Receptors (GPCR)
- cAMP Phosphodiesterase Assay
- Protein Kinase Assay (PK)