Antibiotics
For the convenience of our clients, all antibiotics have been prepared in a final working solution of 25 mg in a volume of 500 ul dispensed in Eppendorf tubes. This is the standard volume to add to 500 ml of culture medium for a final concentration of 50 mg/ml.
Ampicillin: An antibiotic used for the selection of resistant bacteria. Bactericidal to growing cells via inhibition of cell wall biosynthesis. Resistance to ampicillin is mediated by B-lactamase (bla gene) cleavage of the B-lactam ring.
Kanamycin: An antibiotic used for the selection of resistant bacteria. Bactericidal. Binds 70S ribosomal subunit inhibiting translocation and eliciting miscoding. Resistance conferred by KanR-Tn5 gene product which modifies the antibiotic and prevents interaction with ribosomes. Monosulfate from Streptomyces kanamyceticus
Tetracycline: An antibiotic used for the selection of resistant bacteria. Bacteriostatic. Inhibits protein synthesis by preventing binding of aminoacyl-tRNA to ribosome. Resistance conferred by TetR-Tn10 gene product which blocks cell wall permeability.
Zeocin: An antibiotic used for the selection of resistant bacteria, yeast, and mammalian cells. Intercalates into and cleaves DNA.
G418: An aminoglycoside antibiotic commonly used for the selection of mammalian, plant, or yeast cells. G418 interferes with the function of 80S ribosomes and blocks protein synthesis in eukaryotic cells. Resistance conferred by Tn5 or Tn601 gene products.
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