Porcine 9 CFR Testing
MDS offers reliable testing for Porcine pathogens, both in trypsin derived from porcine pancreas and in biological samples previously exposed to trypsin (such as cell lines, bulk harvest, in-process samples, vaccines, etc). The assay complies with Title 9 CFR, part 113.53., Animals and Animal Products.
Clients may also request the testing be modified for certain sample types, or extended, as desired.
Porcine parvovirus (PPV)
To determine whether trypsin samples or cell cultures exposed to trypsin are contaminated with PPV, they are inoculated into porcine cells susceptible to PPV, and maintained for at least 14 days. (This maintenance period may be extended upon request). At least 7 days after inoculation, monolayers from the test cultures are prepared and analyzed using immunofluoresence staining techniques. Alternatively, the samples will be analyzed by PCR
To serve as negative controls, cell cultures inoculated with culture medium are maintained in parallel with test cultures. Similarly, other monolayers are prepared from test cultures at least 7 days after inoculation; these positive controls are inoculated with PPV and likewise maintained in parallel. Reporting time for porcine 9 CFR testing is 4 weeks.
Porcine Circovirus (PCV)
Ciroviruses are considered pathogens in plants, birds and mammals.
PCV, may be the cause of cell culture contaminant. In pigs they have been linked to a variety of symptomatic and asymptomatic illnesses. PCV has been isolated from stillborn piglets, implicating this agent as a possible foetopathogen and pose a serious threat to production.
MDS offers assays for detection of porcine circovirus in
- Trypsin derived from porcine pancreas,
- Biological samples previously exposed to trypsin (such as cell lines, bulk harvest, in-process samples, vaccines, etc)
- Health monitoring of porcine used in high-profile research on xenotransplantation