Rabbit & Rodent Diagnostic Associates

Bovine Pathogens

MDS offers testing of biological samples to detect contamination by specified bovine viruses. This testing plays a vital part in the assaying of raw materials of bovine origin (e.g., bovine serum), cell lines maintained in the presence of bovine raw materials, bulk harvests, in-process samples, vaccines, and so on.
The panel of viruses used in our testing is prescribed in the Code of Federal Regulations (9 CFR, parts 113.46 and 113.47), Animals and Animal Products. Included are the following:

  1. Bovine viral diarrhea virus
  2. Bovine parvovirus
  3. Bovine adenovirus
  4. Bluetongue virus
  5. Bovine respiratory syncytial virus
  6. Reovirus
  7. Rabies virus
  8. Bovine herpes virus I (BHV I)
  9. Bovine herpes virus IV (BHV IV)

Customers may request the inclusion of other viruses, or modification of our standard protocols, as desired.
This testing reliably determines whether a sample is contaminated with any of the above viruses. The assay complies with 9 CFR, part 113.53. The test begins with bovine turbinate (BT) and Vero cells, which are susceptible to a wider range of viruses. These cells are inoculated with the test sample and cultured for at least 21 days.
At least 14 days after inoculation, monolayers are prepared from test cultures for end-point analysis. Samples are analyzed at end point by immunofluorescence, hemadsorption, and cytological staining.
To serve as negative controls, cultures of BT and Vero cells inoculated with culture medium are maintained in parallel with test cultures. Similarly, other monolayers are prepared from test cultures at least 14 days after inoculation; these are inoculated with positive control viruses and likewise maintained in parallel.
Reporting time for bovine 9 CFR testing is 5 weeks.

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