Molecular Diagnostics Services, Inc.
A Contract Research Organization

2-D Gel Electrophoresis

Routine 2-D Gel Service: Routine service includes sample preparation (solubilization), running both dimensions, staining of gels and presenting stained gels and digital images on a disk.

Accepted Sample Submissions Include: Sample Preparation:

Please Note: Sample fractionations are not included in Routine 2-D Service. The costs associated with these procedures are estimated on a case-by-case basis.

2-D Electrophoresis: Following sample preparation, the IEF dimension is performed using 7 cm or 18 cm IPG strips of selected pH range (3-10, 4-7, 3-6, 5-8, 7-10, 6-9, 3-7, 6-11, 3.5-4.5, 4-5, 4.5- 5.5, 5- 6, 5.5- 6.7).

Molecular weight standards are incorporated at the start of the second dimension.

SDS-PAGE for 18 cm strips is performed using pre-cast gradient or fixed percentage, 22 x 22 cm format tricine or tris-glycine gels.

Depending on the protein(s) of interest, 20 % gels are used to resolve small proteins, 12 % gels to resolve mid-range proteins, and 7.5% gels for large proteins. SDS-PAGE for 7 cm strips is performed using Novex NuPAGE 4-12% Bis-Tris ZOOM gels.

Staining of Gels and Acquisition of Digital Images

Three methods are available:
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