Estrogen Receptor Binding and Competitive Binding Assays Using Rat Uterine Cytosol

The Estrogen Receptor (ER) Binding Assay serves as an in vitro screening assay for identifying compounds that can bind to the recombinant human estrogen receptor-α. It is used to determine the ability of a compound to compete with 17β-estradiol for binding to the recombinant human estrogen receptor-α (hrERα)-ligand binding domain (LBD)

This assay is part of the OECD framework for the Testing and Assessment of Endocrine Disrupting Chemicals” under the EPA.