Immunophenotyping
Flow cytometry is commonly used in immunotoxicity studies to assess changes in relative frequency and number of lymphoid and myeloid cells in the spleen, lymph nodes, bone marrow and/or peripheral blood of rodents, and in the peripheral blood of humans. Many of the markers expressed in mammals are highly conserved across species and have been designated as genetic clusters of differentiation (CD).
Selected Markers for Immunophenotyping
Cell Population |
Mouse |
Rat |
Dog |
Primate |
HSC |
CD34 |
|
|
CD34 |
|
SCFR (CD 117) |
|
|
|
Lymphoid Stem Cells |
SCFR (CD 117); IL-7R (CD 127) |
|
|
|
Myeloid Stem Cells |
GCSF-R or GM-CSFR (CDI I4, CDI 15, CDI 16) |
|
|
|
Common Leukocyte |
CD45 |
CD45 |
|
|
Dendritic Cells |
CDI Ic; CD207;CD289; (TLR9) |
CDI lb/c |
|
|
Lymphocytes |
|
|
|
|
Pan-T Cell Marker |
CD3;CD2; Thy 1.1/1.2; TCRI3; CD28 |
CD3 |
CD3; CD5 |
CD3 |
Helper T Cells |
CD4 |
CD4 |
CD4 |
CD4; CD3 |
Cytotoxic T Cells |
CD8 |
CD8 |
CD8 |
CD4; CD3 |
Treg |
FoxP3 |
|
|
|
Activated T Cells |
CD251 (IL-2R); CD69T;CCR7 |
|
|
|
Pan-B Cell Marker |
CD45R1B220; CD19; mlg (ii, i’ a, heavy chain, or K, X; light chain) |
CD45RA |
CD21 |
CD20+/ CD- |
Activated B Cells |
MHC II |
MHC II* |
MHC II* |
MHC II* |
Plasma Cells |
PC-I |
|
|
|
Monocytes/ Macrophages |
CD 14; MHC; UI; CDI1b; (Mac-I); CD282 (TLR2) |
MHC II* |
MHC II* |
MHC II* |
Natural Killer Cells |
NKI .1; CD94; CD251;CD69t; CDl6lc |
CDI6I |
|
CDI 6+/CD3- |
NKT |
CD3 CD56 |
CD3 / DI6I |
|
|