Cell Aging Characterization of MCB
Aged cell banks (16 passages beyond MCB) will be compared with MBC for the following characterization studies:
- Cytogenetic analysis (ploidy)
- Copy number of E1 and E4 insertions
- Chromosome localization of E1 and E4 insertions
- DNA sequence of inserted gene
- Protease sensitivity of inserted gene product
- PCR identity assay for genotyping